Tsis ntev los no, cov ntawv xov xwm tseem ceeb tshaj plaws Kev Tshawb Fawb tau tshaj tawm qhov kev nce qib tshiab ntawm kev sim tshuaj tiv thaiv kab mob qog noj ntshav online. Peb tus neeg mob uas muaj mob qog noj ntshav tau txais kev kho T-cell tshiab ua ke nrog kev hloov kho noob caj noob ces. Nws yog qhov tseem ceeb uas qhov no yog thawj zaug hauv Tebchaws Meskas los tshuaj xyuas kev kho lub xovtooj ntawm tes raws li CRISPR hloov kho cov neeg mob qog noj ntshav.
Qhov kev kho T-cell tshiab no zoo ib yam li cov tsheb-t peb tau hnov ntau zaus. Nws yog los tawm tsam mob qog noj ntshav los ntawm kev hloov tus neeg mob' nws tus kheej lub cev tiv thaiv kab mob. Cov kws tshawb fawb yuav tsum sau T cells los ntawm cov neeg mob' ntshav thiab tso tshuaj kho T cell receptor (TCR) los ntawm kev tshuaj ntsuam genetic engineering hauv vitro txhawm rau txheeb xyuas cov protein zoo NY-ESO-1 ntawm cov qog nqaij hlav cancer. Qhov tshuaj tiv thaiv kab mob qog noj ntshav no muab lub hom phiaj nrog qhov tshwj xeeb siab thiab tsis muaj tshuaj lom thiab muaj kev phiv rau kev kho T cell. Piv nrog kev kho tsheb -t cell, kev kho mob tcr-t cell muaj tsawg dua qhov xwm txheej phom sij rau lub neej xws li cytokine tso tawm cov tsos mob.
Los ntawm T cell kho, crispr-cas9 noob kho tshuab txuas ntxiv muab cov cuab yeej muaj zog los txhim kho lub peev xwm tiv thaiv kab mob qog noj ntshav ntawm T cell.
Ua ntej tso rau hauv TCR, cov kws tshawb fawb tau siv CRISPR thev naus laus zis txhawm rau tshem tawm peb lub noob hauv T cells. Ob ntawm lawv yog TCR noob nqa los ntawm T cells, uas tuaj yeem zam kev ua tsis raug lossis kev sib tw ntawm ntuj TCR thiab kev tsim cov noob caj noob ces tso, thiab txhim kho lub luag haujlwm TCR kho. Qhov thib peb yog lub noob encoding PD-1 receptor. Ib yam li cov tshuaj tiv thaiv kab mob tiv thaiv kab mob uas tiv thaiv kev ua haujlwm ntawm PD-1 protein tuaj yeem pab T cells tshem tawm&"nres; nres &"; cell txo.
Txhawm rau ntsuas qhov ua tau zoo thiab muaj kev nyab xeeb ntawm lub tswv yim no, cov kws tshawb fawb tau ua ntu 1 kev sim tshuaj." Qhov kev sim no feem ntau tsom mus rau peb lo lus nug: peb puas tuaj yeem hloov kho T cells hauv txoj kev tshwj xeeb no? Puas yog T cells tseem ua haujlwm? Cov cell no puas tuaj yeem rov qab siv tau zoo rau tus neeg mob?" hais tias Dr. Edward stadtmauer, tus thawj coj ntawm txoj kev tshawb fawb thiab ua tus saib xyuas kev sim tshuaj." Cov ntaub ntawv thaum ntxov qhia tias lo lus teb rau peb lo lus nug no yog yog."
Hauv cov txiaj ntsig tshaj tawm, T cells kho los ntawm cov noob no tsis ua rau muaj kev phom sij cuam tshuam nrog kev kho mob hnyav, thiab pom kev nyob ntev thiab muaj peev xwm nthuav dav. Ntawm peb tus neeg mob qog noj ntshav koom nrog hauv qhov kev sim, ob leeg yog cov kws kho mob ua rau lub cev puas tsuaj thiab ib tus yog mob qog noj ntshav metastatic sarcoma. Cov txheej txheem kev kho mob uas lawv tau txais yav dhau los tsis muaj txiaj ntsig. Tom qab tau txais T cell reinfusion, tsim kho T cells nrog hloov kho cov noob tseem tuaj yeem kuaj pom hauv cov neeg mob txog li 9 lub hlis. Ntxiv mus, thaum cov kws tshawb fawb cais cov noob caj noob ces hloov kho T cells los ntawm cov neeg mob thiab coj lawv rov qab mus rau chav kuaj sim, lawv pom tias lawv tseem muaj peev xwm tua cov qog nqaij hlav cancer.
& quot; Cov kev tshawb fawb yav dhau los tau qhia tias cov cell no yuav plam lawv txoj haujlwm tsis pub dhau ob peb hnub, tab sis tam sim no cov txiaj ntsig tau qhia tias CRISPR kho cov cell tuaj yeem tswj hwm kev tiv thaiv qog noj ntshav tau ntev tom qab txhaj ib zaug,&"; Xibfwb June hais.
Cov kws tshawb fawb tseem tau hais tias qhov kev soj ntsuam ua ntej tau txais kev nyab xeeb, tab sis nws tseem xav tau cov neeg mob ntxiv thiab soj ntsuam ntev dua ua ntej nws tuaj yeem ntsuas qhov kev nyab xeeb ntawm txoj hauv kev tshiab no. Ib qho ntxiv, txawm hais tias kev hloov kho caj ces ua haujlwm T cells ua haujlwm tau zoo rau mob qog noj ntshav tseem yog cov lus nug tseem ceeb uas yuav tsum tau teb hauv kev tshawb fawb txuas ntxiv.
Cov kws tshawb fawb tau hais tias cov ntaub ntawv tshiab yuav qhib lub qhov rooj rau kev tshawb fawb tom ntej thiab kawm txuas ntxiv seb txoj kev tshiab no tuaj yeem txuas mus rau qhov dav hauv kev kho mob qog noj ntshav. Nws cia siab tias kev hloov kho noob caj noob ces tuaj yeem muaj txiaj ntsig zoo rau cov neeg mob qog noj ntshav yav tom ntej.
