Qhov Kev Tawm Tseg Ntawm Cov Khaub Ncaws Hnav

Nws muaj cuaj hom, uas yog txawv raws li xim ntawm lub kaus mom.

1. Cov kab mob ntshav liab dawb hau, cov hlab ntsha uas tsis siv tshuaj ntxiv, uas siv cov kab mob ntshav qab zib txhua zaus, ntshav txhab ntshav thiab kuaj ntshav.

2. Phem mob ntshav qab hau qhwv lub taub hau, nrog coagulant hauv cov hlab ntsha ntshav, ua kom cov txheej txheem coagulation sai. Cov kab mob sai sai tau coagulate cov ntshav tau txais hauv 5 feeb, uas yog tsim rau kev sim tshuaj ntsuam txog kev mob ceev ceev.

3. Kev sib cais hauv khov yog siv los txhawb lub hau npog lub taub hau, thiab muaj ib qho kev sib cais hauv tsev thiab ib coagulant hauv cov hlab ntshav. Qee zaus tom qab centrifugal inertia sib cais ntawm cov kua nplaum rau cov kua dej hauv cov ntshav, ntshav qab zib thiab ntshav qab zib) thiab cov khib nyiab (cov qe ntshav liab, cov qe ntshav dawb, platelets, thiab fibrin, etc.) tag nrho thiab txuam nrog nruab nrab ntawm lub raj ib qho thaiv, qhov hnoos qeev yog nyob ruaj hauv 48 teev. Coagulant sai sai qhib cov coagulation mechanism thiab accelerate coagulation, uas yog tsim rau thaum muaj xwm ceev kuaj ntshav biochemical.

4. Heparin anti-coagulant tube lub taub hau ntsuab hau, nrog heparin hauv cov hlab ntshav. Heparin muaj cov tshuaj tua kab mob hauv siab thiab muaj peev xwm ua rau cov ntshav khov ntev heev. Nws yog tsim rau kev kuaj ntshav erythrocyte brittleness, roj ntshav tsom, hematocrit kuaj, ntshav qias thiab probiosis kuaj, tsis haum rau cov ntshav coagulation xeem. Heparin ntau dhau yuav ua rau cov leukocyte aggregation, thiab siv tsis tau rau hauv cov ntshav dawb. Nws tsis haum rau kev cais ntawm cov qe ntshav dawb vim tias nws tuaj yeem ua rau tom qab lub teeb xiav tom qab dyeing.

5. Plasma separation tube lub teeb ntsuab epicranium, nyob rau hauv qhov kev sib koom ua ke sib txig ua ke nrog cov lithium heparin anticoagulant, muaj peev xwm ua tau lub hom phiaj ntawm kev sib cais ntawm cov ntshav, yog qhov kev xaiv zoo tshaj plaws ntawm lub ntsha nrhiav, kuj siv tau rau kev kuaj ntshav cov ntshav niaj zaus thiab ICU thaum muaj xwm ceev plasma biochemical detection, etc. Cov plasma specimen tuaj yeem ncaj qha thiab ntsia ruaj khov rau 48 teev hauv tub yees.

6. EDTA anticoagulant tube purple epicranium, ethylenediamine tetraacetic acid (EDTA, molecular weight 292) thiab nws ntsev yog ib hom ntawm, ua tau zoo chelate calcium ions nyob rau hauv cov ntshav kuaj, chelating calcium los yog calcium tshuaj yuav tsiv mus rau thaiv thiab tshem tawm cov kev phais lossis cov tshuaj phiv heev, ua kom tsis txhob muaj ntshav khov ntshav. Siv cov tshuaj tua kabmob (Hematology), tsis muaj cov kev kuaj ntshav thiab kuaj platelet, tseem tsis siv rau calcium, potassium, sodium, hlau, alkaline phosphatase, thiab kev txiav txim ntawm creatine kinase thiab leucine aminopeptidase, haum rau PCR kuaj.

7. Ntsuas dej citrate coagulation test tube lub teeb xiav taub hau, sodium citrate yog siv los ntsuas cov tshuaj calcium uas nyob hauv cov ntshav. Ua raws li cov kev sim tshuaj, ua haujlwm hauv lub teb chaws yuav tsum kuaj cov qauv kev soj ntsuam (NCCLS) yog 3.2% lossis 3.8% (sib npaug 0.109 mol / L lossis 0.129 mol / L), cov tshuaj anticoagulants thiab ntshav piv ntawm 1: 9.

8. Sodium citrate blood sedimentation test tube roj cranium, qhov concentration ntawm sodium citrate los ntawm kev kuaj ntshav ntshav yog 3.2% (sib npaug 0.109mol / L) thiab qhov piv txwv ntawm anticoagulant rau cov ntshav yog 1: 4.

9. Phosphorus oxalate / sodium fluoride grey taub hau hau. Sodium fluoride yog ib qho tsis muaj zog anticoagulant. Nws feem ntau siv nrog potassium oxalate los yog sodium iodate, nrog ib qho piv txwv ntawm ib qho sodium fluoride thiab peb kab poov tshuaj oxalate. Qhov sib tov ntawm 4 mg tuaj yeem ua 1 ml ntshav qab zib nyob rau hauv 23 hnub kom ua kom zoo thiab suppress decomposition, nws yog ib qho kev txiav txim siab zoo ntshav qab zib rau cov tshuaj txhuam, tsis tuaj yeem siv los txiav txim siab ntawm urea enzymatic urea, tseem tsis tau siv rau kev txiav txim ntawm alkaline phosphatase thiab amylase, pom zoo rau kev ntsuas ntshav qabzib.